Microorganisms in Refrigerated Storage and Freezing

Survival of microorganisms after ultrarapid freezing and thawing.

Numerous reports during the past 75 years have described, usually in qualitative terms, the survival of various bacteria, yeasts, and fungi after exposure to very low temperatures (Smith, Biological Effects of Freezing and Supercooling, p. 74-137, The Williams & Wilkins Co., Baltimore, 1961). Although rates of cooling and warming have been shown to affect survival quantitatively (Mazur, Ann. N....

Manipulation of Storage Polysaccharides in Microorganisms

Effect of Padinaaustralis Alga Extract on Fat Oxidative Degradation in Epinepheluscoioides during Refrigerated Storage

Background and Objectives: Recently, the use of synthetic antioxidants has been limited due to having chemical and toxic compounds, so the use of natural compounds such as algae, as an alternative to synthetic compounds, is important for the storage of fishery products. The aim of this study was to investigate the effect of Padina australis alga extract on controlling the oxidative spoilage of ...

Safety of refrigerated storage of admixed parenteral fluids.

Many hospital pharmacies are reluctant to store admixed parenteral fluids longer than 24 to 48 h because of concern about possible microbial contamination. We evaluated the safety of prolonged refrigerated storage of admixtures by culturing mixtures in 471 bags prepared routinely in a hospital pharmacy and stored at 4 degrees C for up to 15 days. Low-level contamination (1 CFU per bag) was foun...

Impact of storage solution formulation during refrigerated storage upon chondrocyte viability and cartilage matrix.

Various preservation solutions have been evaluated for longer hypothermic cartilage storage for tissue transplantation; however, the results are mixed. This research was carried out to determine whether phosphate-buffered saline (PBS) or organ preservation solutions would preserve both the extracellular matrix and chondrocytes of articular cartilage better than culture medium during refrigerate...